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Hepatitis B

ELISA test system Vitrotest

Hepatitis B virus (HBV) is an enveloped DNA virus of the family Hepadnaviridae. The aetiology of ‘‘serum hepatitis’’, as it was known for many years, was not identified, until the discovery of the (...) continue »

Vitrotest HBsAg TK016

ELISA test system «Vitrotest HBsAg» is intended for detection of surface antigen of HBV (HBsAg) in human serum or plasma.

Detection of antibodies is based on the principle of one-step "sandwich"-variant enzyme immunoassay (ELISA) using a pair of monoclonal antibodies.

  • Variant of complectation: 96-1T (12xS8-1T) or 192-1T (24xS8-1T).
  • Solid phase - ELISA plate with immobilized monoclonal antibodies specific for HBsAg.
  • Conjugate: monoclonal antibodies specific for HBsAg conjugated with horseradish peroxidase.
  • Chromogen: one-component TMB.
  • Volume of serum for analysis: 100 mcl.
  • Timing Analysis - 2.5 hours (shaker-incubator).

Vitrotest HBsAg-Confirmation TK017

Set of reagents «Vitrotest HBsAg-Confirmation» intended to confirm the presence of the surface antigen of hepatitis B virus HBsAg in human serum or plasma. The kit used with ELISA test system «Vitrotest HBsAg».

The method is based on the neutralization of HBsAg, that is in serum or plasma, using in the analysis specific monoclonal antibodies to HBsAg.

The composition of the set: Set includes a sample diluent and serum neutralization solution (monoclonal antibodies specific for HBsAg).

Vitrotest Anti-HBcore TK018

ELISA test system «Vitrotest Anti-HBcore» designed to detect total antibodies to the hepatitis B virus core antigen in serum or plasma.

Detection of antibodies based on the principle of competitive one-step solid-phase enzyme immunoassay.

  • Variant of complectation: 96-1T (12xS8-1T).
  • Solid phase - ELISA plate with immobilized recombinant HBcore antigen.
  • Conjugate - anti-species (specific for human IgG and IgM) monoclonal antibodies conjugated to horseradish peroxidase.
  • Chromogen - one-component TMB.
  • Volume of serum for analysis - 20 mcl.
  • Timing Analysis - 1.5 - 2 hours.

Vitrotest HBcore-IgM TK019

ELISA test system «Vitrotest HBcore-IgM» is intended for detection of IgM class antibodies to Hepatitis B virus core antigen in human serum or plasma.

Detection of antibodies is based on the principle of "IgM-capture" solid phase enzyme immunoassay (ELISA).

  • Variant of complectation: 96-1T (12xS8-1T).
  • Solid phase - ELISA plate with immobilized monoclonal antibodies specific for human IgM immunoglobulin.
  • Conjugate: HBcore recombinant antigen conjugated with horseradish peroxidase.
  • Chromogen: one-component TMB.
  • Volume of serum for analysis: 10 mcl.
  • Timing Analysis - 1.5 - 2 hours.

Vitrotest HBcore-IgG TK 050

ELISA test system «Vitrotest HBcore-IgG» is intended for detection of IgG class antibodies to core antigen of Hepatitis B virus in human serum or plasma.

Detection of antibodies is based on the principle of "indirect" solid phase enzyme immunoassay (ELISA).

  • Variant of complectation: 96-1T (12xS8-1T).
  • Solid phase - ELISA plate with immobilized recombinant HBcore antigen of Hepatitis B virus/
  • Conjugate: anti-species (specific for human IgG) monoclonal antibodies conjugated with horseradish peroxidase.
  • Chromogen: one-component TMB.
  • Volume of serum for analysis: 20 mcl.
  • Timing Analysis - 1.5 - 2 hours.

Vitrotest Anti-HBs TK020

ELISA test system «Vitrotest Anti-HBs» designed to detect antibodies to the surface antigen of hepatitis B virus in human serum or plasma.

Detection of antibodies based on the principle of "sandwich" solid-phase enzyme immunoassay (ELISA).

  • Variant of configuration: 96-1T (12xS8-1T).
  • Solid phase - ELISA plate with immobilized surface antigen of Hepatitis B virus
  • Conjugate - recombinant proteins, analogues of HBsAg subtypes ad and ay conjugated to horseradish peroxidase.
  • Chromogen - one-component TMB.
  • Volume of serum for analysis - 50 mcl.
  • Timing Analysis - 2.5 hours.

Vitrotest Calibrators Anti-HBs TK021

Set of calibrators «Vitrotest Calibrators Anti-HBs» is intended for the quantitative determination of the concentration of antibodies to the surface antigen of hepatitis B virus - HBsAg - in human serum or plasma.

The kit used with ELISA test system «Vitrotest Anti-HBs» for constructing the calibration graph and quantifying the results of determining the concentration of antibodies to HBsAg.

The composition of the set: 5 calibrators with different concentrations of antibodies specific to HBsAg: 0 mIU/ml, 10 mIU/ml, 50 mIU/mL, 100 mIU/ml, 200 mIU/ml.